Approximate Work Schedule for Implementation of Activities Under the Project

 

Year 1

Year 2

Year 3

ACTIVITY

Q1

Q2

Q3

Q4

Q1

Q2

Q3

Q4

Q1

Q2

Q3

Q4

Workpackage 1

Develop top-crosses in Brazil

 

 

 

 

 

 

 

 

 

 

 

 

Top-cross evaluation in Brazil, Cameroon, Guadeloupe, Colombia

 

 

 

 

 

 

 

 

 

 

 

 

Synthetic obtainted

 

 

 

 

 

 

 

 

 

 

 

 

Diallel obtained

 

 

 

 

 

 

 

 

 

 

 

 

Diallel evaluation

 

 

 

 

 

 

 

 

 

 

 

 

Evaluation in soil and nutrient solution of aluminium RILs

 

 

 

 

 

 

 

 

 

 

 

 

Obtention of RILS for P efficiency

 

 

 

 

 

 

 

 

 

 

 

 

 

Workpackage 2

Management strategies on soil chemical, biological and physical characteristics, remnant effect of soil management

 

 

 

 

 

 

 

 

 

 

 

 

Soil characterisation

 

 

 

 

 

 

 

 

 

 

 

 

Identification of appropriate cropping system and soil management practices adapted to farmer conditions

 

 

 

 

 

 

 

 

 

 

 

 

Parameter of crop model

 

 

 

 

 

 

 

 

 

 

 

 

Technical  itineraries and simulation tool

 

 

 

 

 

 

 

 

 

 

 

 

 

Workpackage 3

 Correlation between root tip exudation of organic acids, the accumulation of Al in root tips & tolerance between contrasting genotypes

 

 

 

 

 

 

 

 

 

 

 

 

 Spatial location of AL in maize root tips and the interaction of time of exposure x level of Al x tolerance differential

 

 

 

 

 

 

 

 

 

 

 

 

Modification of rhizosphere pH and Al resistance differential between contrasting maize genotypes

 

 

 

 

 

 

 

 

 

 

 

 

Protein patterns in root tips and root exudates

 

 

 

 

 

 

 

 

 

 

 

 

 

Workpackage 4

Eight lines evaluated for acid organic secretion in aluminium and phosphorus stresses

 

 

 

 

 

 

 

 

 

 

 

 

Crosses among eight inbred lines and a line with over-expression of citrate synthase

 

 

 

 

 

 

 

 

 

 

 

 

Evaluation of crosses in field and lab

 

 

 

 

 

 

 

 

 

 

 

 

 

Workpackage 5

Pectin contents

 

 

 

 

 

 

 

 

 

 

 

 

Degree of methylation

 

 

 

 

 

 

 

 

 

 

 

 

The role of the Zmal1 gene product on Al resistance

 

 

 

 

 

 

 

 

 

 

 

 

 

Workpackage 6

Surface negativity

 

 

 

 

 

 

 

 

 

 

 

 

Protoplast viability

 

 

 

 

 

 

 

 

 

 

 

 

Membrane-lipid peroxidation

 

 

 

 

 

 

 

 

 

 

 

 

Enzyme activities related to oxidative stress

 

 

 

 

 

 

 

 

 

 

 

 

Omt134-expreessing plants in response to Al

 

 

 

 

 

 

 

 

 

 

 

 

ZMGST2 coded proteins

 

 

 

 

 

 

 

 

 

 

 

 

 

Workpackage 7

  Advance one generation of self pollination to develop the F6:7 RILs

 

 

 

 

 

 

 

 

 

 

 

 

Select RFLP and SSR markers against the parental lines and contrasting DNA bulks

 

 

 

 

 

 

 

 

 

 

 

 

 Saturated genetic maps with markers associated to aluminium tolerance developed

 

 

 

 

 

 

 

 

 

 

 

 

 QTLs characterized in relation to theirs effect and action

 

 

 

 

 

 

 

 

 

 

 

 

 

Workpackage 8

Characterization of phosphate transporters-genes induced in P-starved maize roots

 

 

 

 

 

 

 

 

 

 

 

 

Characterization of maize genetic standards for contrasting phosphate-transporters genes

 

 

 

 

 

 

 

 

 

 

 

 

Characterization of contrasting phosphate-transporter genes and DNA sequence

 

 

 

 

 

 

 

 

 

 

 

 

 

Workpackage 9

Cloning of the ZMGST2 promoter

 

 

 

 

 

 

 

 

 

 

 

 

Expression of the Zmal1 promoter in maize

 

 

 

 

 

 

 

 

 

 

 

 

Analysis of promotors active in the roots of trangenic maize plants

 

 

 

 

 

 

 

 

 

 

 

 

Analysis of the Zmal1 promoter in transgenic tobacco

 

 

 

 

 

 

 

 

 

 

 

 

Characterisation of transgenic maize plants expressing genes under the control of promotors active in roots

 

 

 

 

 

 

 

 

 

 

 

 

Expression of the Zmgst2:GUS in maize

 

 

 

 

 

 

 

 

 

 

 

 

 

Workpackage 10

Writing and calibration of the model

 

 

 

 

 

 

 

 

 

 

 

 

Experimental measurement in the rhizosphere

 

 

 

 

 

 

 

 

 

 

 

 

Validation of the model

 

 

 

 

 

 

 

 

 

 

 

 

 

Workpackage 11

Isolation and characterisation of native AM fungi from acid soils

 

 

 

 

 

 

 

 

 

 

 

 

Characterisation of the contribution of these AM fungal strains to maize plant adaptation to soils acidity

 

 

 

 

 

 

 

 

 

 

 

 

Study of the role of AM fungal strains in Al resistance of maize