The group in the Institut de Biologia Molecular de Barcelona, CSIC, has been working in the molecular biology of maize during the last 15 years. A number of genes from this species have been described in the group including four genes coding for alpha-tubulin, the gene coding for a major protein component of the cell wall (HRGP), the genes coding for enzyme of the lignin biosynthesis pathway or a malic enzyme specifically expressed in the embryo root epidermis. The group has participated co-ordinating a previous INCO proposal on aluminium tolerance and it has experience in the analysis of genes promoters both in maize and in model plants such as tobacco. It has also been identifying the structure and expression of genes in maize by a number of techniques including in situ hybridization.
Lopez Becerra, E., P. Puigdomenech & V. Stiefel: A Gene Coding for a Malic Enzyme Expressed in the Embryo Root Epidermis from Zea mays (Accession No. AJ224847). Plant Physiol. (1998) 127, 331-334.
Uribe, X., M.A. Torres, M. Capellades, P. Puigdomènech & J. Rigau: Maize "-tubulin genes are expressed according to specific patterns of cell differentiation. Plant Mol. Biol. (1998) 37, 1069-1078.
Menossi, M., J.A. Martínez-Izquierdo & P. Puigdomènech: Promoter tissue specific activity and ethylene control of the gene coding for the maize hydroxyproline-rich glycoprotein in maize cells transformed by particle bombardment. Plant Science (1997) 125, 189-200.
Capellades, M., M.A. Torres, I. Bastisch, V. Stiefel, F. Vignols, W.R. Bruce, D. Peterson, P. Puigdomènech & J. Rigau: The maize caffeic acid O-methyltransferase gene promoter is active in transgenic tobacco and maize plants. Plant Mol. Biol. (1996) 31, 307-322
Bonfante, P., R. Bergero, X. Uribe, C. Romera, J. Rigau & P. Puigdomènech: Transcriptional activation of a maize "-tubulin gene in mycorrhizal maize and transgenic tobacco. Plant J. (1996) 9, 737-743.